In chromatography, which option best describes Column Chromatography where components separate as they pass through a column?

In chromatography, which option best describes Column Chromatography where components separate as they pass through a column?

• A. Chromatography
• B. Paper Chromatography
• C. Column Chromatography
• D. Diafiltration

Answer: (C)

Column Chromatography is shown here. In this technique, the mixture is applied to a column packed with a stationary phase, and a mobile phase flows through it. The components separate because they interact differently with the stationary phase, moving at different speeds as they pass through the column. This creates distinct bands that elute at different times, exactly the situation described. The other options refer to different methods—Paper Chromatography uses paper as the stationary phase, and Diafiltration is a membrane-based filtration process—not a column-based separation. The general term Chromatography covers many methods, but the description in question points to the column-based approach.

Column Chromatography is shown here. In this technique, the mixture is applied to a column packed with a stationary phase, and a mobile phase flows through it. The components separate because they interact differently with the stationary phase, moving at different speeds as they pass through the column. This creates distinct bands that elute at different times, exactly the situation described. The other options refer to different methods—Paper Chromatography uses paper as the stationary phase, and Diafiltration is a membrane-based filtration process—not a column-based separation. The general term Chromatography covers many methods, but the description in question points to the column-based approach.